研究業績
プレスリリース
(2024) プロポリス主成分の微生物生産における世界最高値を10倍以上更新
(2024) ダイズ根圏細菌のイソフラボン代謝遺伝子クラスターを発見―根圏形成メカニズムの理解や有用物質生産に貢献―
(2023) トマト根に定着する細菌からトマトの毒を分解する酵素を発見―土壌微生物が植物の分泌する有害成分を解毒するメカニズムの理解に貢献―
(2023) 日本の伝統文化と植物科学を結ぶ「紫」の糸―絶滅危惧植物ムラサキをめぐる昔と今―
(2023) ダイズ根圏へのイソフラボン供給量を増やす酵素を発見―植物が機能性成分を根から土壌へ分泌するメカニズムの理解に貢献―
(2021) トマトのトマチンによる根圏細菌叢の制御 ?トマト苦味・有毒物質の根圏での新しい機能?
(2020) ダイズ根圏に殺虫活性物質オカラミンを発見 －土の中の遺産「根圏ケミカル」をメタボローム解析で明らかに－
(2020) ダイズの分泌物「ダイゼイン」が根圏微生物叢を形成することを解明 －根から数ミリの土壌で働く植物特化代謝物－
(2019) アルテピリンC合成酵素の発見とその生産 －雑草の遺伝子から生理活性物質の生産へ－
学術論文
2024
- Han, J., Munakata, R., Takahashi, H., Koeduka, T., Kubota, M., Moriyoshi, E., Hehn, A., Sugiyama, A., Yazaki, K.
Catalytic mechanism underlying the regiospecificity of coumarin-substrate transmembrane prenyltransferases in Apiaceae.
Plant Cell Physiol., in press - Bamba, T., Munakata, R., Ushio, Y., Kumokita, R., Tanaka, S., Hori, Y., Kondo, A., Yazaki, K., Hasunuma, T.
De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii.
ACS Synthetic Biology, in press - Fujimatsu, T., Tsuno, Y., Oonishi, A., Yano, T., Maeda, H., Endo, K., Yazaki, K., Sugiyama, A.
O-Methylated Isoflavones induce nod genes of Mesorhizobium ciceri and pratensein promotes nodulation in chickpea.
J. Agri. Food Chem. 72(33): 18465-18477 (2024) - Munakata, R. et Yazaki, K.
How did plants evolve the prenylation of specialized phenolic metabolites by means of UbiA prenyltransferases?
Current Opinion in Plant Biology 81: (2024) - Aoki, N., Shimasaki, T., Yazaki, W., Sato, T., Nakayasu, M., Ando, A., Kishino, S., Ogawa, J., Masuda, S., Shibata, A., Shirasu, K., Yazaki, K., Sugiyama, A.
An isoflavone catabolism gene cluster underlying interkingdom interactions in the soybean rhizosphere.
ISME Communications 4: 052 (2024) - Nakanishi, K., Li, H., Ichino, T., Tatsumi, K., Osakabe, K., Watanabe, B., Shimomura, K., Yazaki, K.,
Peroxisomal 4-coumaroyl-CoA ligases participate in shikonin production in Lithospermum erythrorhizon,
Plant Physiol., 195 (4): 2843-2859 (2024) - Oshikiri, H., Li, H., Yamamoto, H., Yazaki, K., Takanashi K.,
Comparative analysis of shikonin and alkannin acyltransferases reveals their functional conservation in Boraginaceae,
Plant Cell Physiol., 65(3): 362-371(2024).
2023
- Takamatsu, K., Toyofuku, M., Okutani, F., Yamazaki, S., Nakayasu, M., Aoki, Y., Kobayashi, M., Ifuku, K., Yazaki, K., Sugiyama, A.
α-Tomatine gradient across artificial roots recreates the recruitment of tomato root-associated Sphingobium Plant Direct 7: e550. (2023) - Nakayasu, M., Takamatsu, K., Kanai, K., Masuda, S., Yamazaki, S., Aoki, Y., Shibata, A., Suda, W., Shirasu, K., Yazaki, K., Sugiyama, A.
Tomato root-associated Sphingobium harbors genes for catabolizing toxic steroidal glycoalkaloids.
mBio 00599-23 (2023) - Sugiyama, A.
Application of plant specialized metabolites to modulate soil microbiota
Plant Biotechnology 40 (2):123-133 (2023) - Ito, E., Munakata, R., Yazaki, K.
Gromwell, a purple link between traditional Japanese culture and plant science.
Plant and Cell Physiology 64(6): 567-570 (2023) - Matsuda, H., Yamazaki, Y., Moriyoshi, E., Nakayasu, M., Yamazaki, S., Aoki, Y., Takase, H., Okazaki, S., Nagano, A. J., Kaga, A., Yazaki, K., Sugiyama, A.
Apoplast-localized β-Glucosidase Elevates Isoflavone Accumulation in the Soybean Rhizosphere.
Plant and Cell Physiology 64: 486?500 (2023) Editor's Choice - Tatsumi, K., Ichino, T., Isaka, N., Sugiyama, A., Moriyoshi, E., Okazaki, Y., Higashi, Y., Kajikawa, M., Tsuji, Y., Fukuzawa, H., Toyooka, K., Sato, M., Ichi, I., Shimomura, K., Ohta, H., Saito, K., Yazaki, K.
Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin.
Journal of Experimental Botany 74:104-117 (2023) - Nakayasu, M., Takamatsu, K., Yazaki, K., Sugiyama, A.
Plant specialized metabolites in the rhizosphere of tomatoes: secretion and effects on microorganisms.
Bioscience, Biotechnology, and Biochemistry 87:13-20 (2023)
2022
- Kiyoto, S., Ichino, T., Awano, T., Yazaki, K.
Improved chemical fixation of lipid-secreting plant cells for transmission electron microscopy. Microscopy 71:206-213 (2022) - Li H., Matsuda H., Tsuboyama A., Munakata R., Sugiyama A., Yazaki K.
Inventory of ATP-binding cassette proteins in Lithospermum erythrorhizon as a model plant producing divergent secondary metabolites.
DNA Research 29. (2022) - Onodera, T., Miyazaki, H., Nakayasu, M., Sakurai, N., Sugiyama, A. . Visualization of Root Exudates from Pea Roots Using Sheets with Immobilized Bovine Serum Albumin. In IEEJ Transactions on Sensors and Micromachines (142:133?138 (2022)
- Onodera T., Miyazaki H., Li X., Wang J., Nakayasu M., Yatabe R., Tahara Y., Hosoki A., Sakurai N., Sugiyama A.
Development of two-dimensional qualitative visualization method for isoflavones secreted from soybean roots using sheets with immobilized bovine serum albumin.
Biosensors and Bioelectronics 196:113705. (2022) - 杉山暁史
根圏微生物叢形成を調節する植物特化代謝産物
植物の生長調節 Vol.57, No.1, 25-31 (2022) - 杉山暁史, 中安大
【植物と共生 すぐそこにある分子】 サポニンを介した 植物と根圏微生物の相互作用
現代化学 6月号 (2022) - 杉山暁史, 小野寺武
ダイズ根圏イソフラボンの測定と可視化
アグリバイオ 6月号 (2022) - 中安大, 杉山暁史
根圏微生物叢を制御するバイオスティミュラント
バイオスティミュラントハンドブック (2022)
2021
- Nakayasu, M., Yamazaki, S., Aoki, Y., Yazaki, K., Sugiyama, A.
(2021) Triterpenoid and Steroidal Saponins Differentially Influence Soil Bacterial Genera.
Plants 10, 2189. (2021) - Shimada, N., Munekata, N., Tsuyama, T., Matsushita, Y., Fukushima, K., Kijidani, Y., Takabe, K., Yazaki, K., Kamei, I.
Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo.
Plants 10:2237. (2021) - Nakayasu, M., Ikeda, K., Yamazaki, S., Aoki, Y., Yazaki, K., Washida, H., Sugiyama, A.
Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field.
Agronomy 11, 1375. (2021) - Koeduka, T., Takarada, S., Fujii, K., Sugiyama, A., Yazaki, K., Nishihara, M., Matsui, K.
Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants.
Metabolic Engineering Communications. 13, e00180. (2021) - Yazaki, W., Shimasaki, T., Aoki, Y., Masuda, S., Shibata, A., Suda, W., Shirasu, K., Yazaki, K., Sugiyama, A.
Nitrogen deficiency-induced bacterial community shifts in soybean roots.
Microbes and Environment. 36(3), (2021) - Sugiyama, A.
(2021). Flavonoids and saponins in plant rhizospheres: roles, dynamics, and the potential for agriculture.
Bioscience, Biotechnology, and Biochemistry. 85(9), 1919-1931 (2021) - Shimasaki, T., Masuda, S., Garrido-Oter, R., Kawasaki, T., Aoki, Y., Shibata, A., Suda, W., Shirasu, K., Yazaki, K., Nakano, R. T., Sugiyama, A.
Tobacco Root Endophytic Arthrobacter Harbors Genomic Features Enabling the Catabolism of Host-Specific Plant Specialized Metabolites.
MBio. e00846-21 (2021) - Munakata R., Olry A., Takemura T., Tatsumi K., Ichino T., Villard C., Kageyama J., Kurata T., Nakayasu M., Jacob F., Koeduka T., Yamamoto H., Moriyoshi E., Matsukawa T., Grosjean J., Krieger C., Sugiyama A., Mizutani M., Bourgaud F., Hehn A., Yazaki K.
Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants.
Proceedings of the National Academy of Sciences 118:e2022294118 (2021) - Yamazaki, S., Mardani-korrani, H., Kaida, R., Ochiai, K., Kobayashi, M., Nagano, A. J., Fujii, Y., Sugiyama, A., Aoki, Y.
Field multi-omics analysis reveals a close association between bacterial communities and mineral properties in the soybean rhizosphere.
Scientific Reports 11 (1), 1-16 (2021) - Savadogo E.H., Shiomi Y., Yasuda J., Akino T., Yamaguchi M., Yoshida H., Umegawachi T., Tanaka R., Suong D.N.A., Miura K., Yazaki K., Kitajima S.
Gene expression of PLAT and ATS3 proteins increases plant resistance to insects.
Planta (in press) - Nakayasu, M., Ohno, K., Takamatsu, k., Aoki, Y., Yamazaki, S., Takase, H., Shoji, T., Yazaki, K., Sugiyama, A.
Tomato roots secrete tomatine to modulate the bacterial assemblage of the rhizosphere.
Plant Physiology 186 (1), 270-284 (2021) - Toyofuku, M., Okutani, F., Nakayasu, M., Hamamoto, S., Takase, H., Yazaki, K., Sugiyama, A.
Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture.
Bioscience, Biotechnology, and Biochemistry 85 (5), 1165-1169 (2021)
2020
- Matsuda, H., Nakayasu, M., Aoki, Y., Yamazaki, S., Nagano, A.J., Yazaki, K., Sugiyama, A.
Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots.
Plant Direct 4 (11), e00286 (2020) - Fujimatsu, T., Endo, K., Yazaki, K., Sugiyama, A.
Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition.
Plant Direct 4 (9), e00259 (2020) - Yamamoto, H., Tsukahara, M., Yamano, Y., Wada, A., Yazaki, K.
Alcohol dehydrogenase activity converts 3"-hydroxygeranylhydroquinone to an aldehyde intermediate for shikonin and benzoquinone derivatives in Lithospermum erythrorhizon
Plant and Cell Physiology 61 (10), 1798-1806 (2020) - Oshikiri, H., Watanabe, B., Yamamoto, H., Yazaki, K., Takanashi, K.
Two BAHD acyltransferases catalyze the last step in the shikonin/alkannin biosynthetic pathway.
Plant Physiology 184 (2), 753-761 (2020) - Izuishi Y., Isaka N., Li H., Nakanishi K., Kageyama J., Ishikawa K., Shimada T., Masuta C., Yoshikawa N., Kusano H., Yazaki K.
Apple latent spherical virus (ALSV)-induced gene silencing in a medicinal plant, Lithospermum erythrorhizon.
Scientific Reports 10 (2020) - Ueoka H., Sasaki K., Miyawaki T., Ichino T., Tatsumi K., Suzuki S., Yamamoto H., Sakurai N., Suzuki H., Shibata D., Yazaki K.
A Cytosol-Localized Geranyl Diphosphate Synthase from Lithospermum erythrorhizon and Its Molecular Evolution.
Plant Physiology 182:1933?1945. (2020) - Tatsumi K., Ichino T., Onishi N., Shimomura K., Yazaki K.
Highly efficient method of Lithospermum erythrorhizon transformation using domestic Rhizobium rhizogenes strain A13.
Plant Biotechnology 37:39?46. (2020) - Okutani F., Hamamoto S., Aoki Y., Nakayasu M., Nihei N., Nishimura T., Yazaki K., Sugiyama A.
Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community.
Plant, Cell & Environment 43:1036?1046. (2020) - Sakurai N., Mardani-Korrani H., Nakayasu M., Matsuda K., Ochiai K., Kobayashi M., Tahara Y., Onodera T., Aoki Y., Motobayashi T., Komatsuzaki M., Ihara M., Shibata D., Fujii Y., Sugiyama A.
Metabolome analysis identified okaramines in the soybean rhizosphere as a legacy of hairy vetch
Frontiers in Genetics 11 (2020) - Hiroshi Teramura, Kazuma Yamada, Kahori Ito, Keisuke Kasahara, Tsubasa Kikuchi, Naoya Kioka, Masato Fukuda, Hiroaki Kusano, Katsunori Tanaka,
Hiroaki Shimada
Characterization of novel SUMO family genes in the rice genome
Genes and Genetic Systems (2020) - Nonoka Sato, Miho Kihira, Ryoko Matsushita, Chihiro Kaneko, Yohei Ishii, Yong-Gen Yin, Naoki Kawachi, Hiroshi Teramura, Hiroaki Kusano, Hiroaki
Shimada
AtFLL2, a member of the FLO2 gene family, affects the enlargement of leaves at the vegetative stage and facilitates the regulation of carbon metabolism and flow
Bioscience, Biotechnology, and Biochemistry (2020) - Chika Honda, Kaoru Ohkawa, Hiroaki Kusano, Hiroshi Teramura, Hiroaki Shimada
A simple method for in planta tomato transformation by inoculating floral buds with sticky Agrobacterium suspension
Plant Biotechnology (2020) - Rintaro Suzuki, Tomohiro Imamura, Yoko Nonaga, Hiroaki Kusano, Hiroshi Teramura, Ken-taro Sekine, Tetsuro Yamashita, Hiroaki Shimada
A novel FLOURY ENDOSPERM2 (FLO2)-interacting protein, is involved in maintaining fertility and seed quality in rice
Plant Biotechnology (2020) - 杉山暁史, 中安?大
植物二次代謝産物による根圏微生物叢の形成と制御
バイオサイエンスとインダストリー VOL.78 NO.4 (2020) - 棟方涼介, 矢崎一史
プロポリスの生理活性物質アルテピリンC合成系の酵母内再構築
バイオサイエンスとインダストリー VOL.78 NO.3 (2020) - 杉山暁史
植物を守る根圏代謝物 -植物により形成される根圏微生物叢の一機能
日本農薬学会誌 45巻2号, 160-161 (2020) - 松田一彦, 櫻井望, 藤井義晴, 杉山暁史
根圏における植物の間接誘導防衛機構の最前線 植物根圏共生概念のリモデリング
化学と生物 58巻6号, 325-329 (2020)