Research

Visualization of reducing ends of cellulose and chitin – substrate specificity of hydrolytic enzymes

We developed a novel method for reducing end electron lavelling to visualize them under TEM. This allows us to see unambiguously how the hydrolytic enzymes such as CBH I, CBH II, Chitinase A, Chitinase B, and other processive enzymes degrade the corresponding substrates.

Kim N-H, Imai T, Wada M, Sugiyama J: Molecular directionality in cellulose polymorphs. Biomacromolecules, 7, 274-280, 2006
Hult E-L, Katouno F, Uchiyama T, Watanabe T, Sugiyama J: Molecular directionality in crystalline β-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170. Biochem J, 388, 851-856, 2005
Imai T, Watanabe T, Yui T, Sugiyama J: Directional degradation of β-chitin by chitinase A1 revealed by a novel reducing end labelling technique. FEBS Lett, 510, 201-205, 2002
Imai T, Boisset C, Igarashi K, Samejima M, Sugiyama J: Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose. FEBS Lett, 432, 113-116, 1998

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